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sirna targeting ctmp  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology sirna targeting ctmp
    Figure 1. Localization of <t>CTMP</t> in mitochondria. (A) Cytoplasm and mitochondria fractions were isolated from A549 cells. Total lystates, the cytosolic fraction, and mitochondria were analyzed by Western blotting (COXIV-mitochondria marker and α-tubulin-cytosolic marker). (B) A549 cells were transiently co-transfected with pEGFP-N1-CTMP and pDsRed-Mito for 24 h, and cells were observed using confocal microscopy. (C) A549 cells stably expressing pDsRed-Mito were infected with lentivirus-CTMP for 24 h and immunostained with anti-CTMP antibody. (D) The mitochondria fraction from A549 cells was isolated and treated with the indicated reagents. Samples were separated into supernatant (S) and precipitate (P) fractions, and then analyzed by immunoblotting with the indicated antibodies (cytochrome c, mitochondria membrane space protein; and COXIV, mitochondria membrane protein). (E) Immunofluorescence assay of apoptosis-inducing factor (AIF). A549 cells were grown on glass coverslips and transiently transfected with pEGFP-N1-CTMP. The cells were then stained with anti-AIF antibody (scale bar, 10 µm).
    Sirna Targeting Ctmp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sirna+targeting+ctmp/CTMP+siRNA/pm22200884-45-12-25
    Average 90 stars, based on 2 article reviews
    sirna targeting ctmp - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Carboxyl-terminal modulator protein induces apoptosis by regulating mitochondrial function in lung cancer cells."

    Article Title: Carboxyl-terminal modulator protein induces apoptosis by regulating mitochondrial function in lung cancer cells.

    Journal: International journal of oncology

    doi: 10.3892/ijo.2011.1319

    Figure 1. Localization of CTMP in mitochondria. (A) Cytoplasm and mitochondria fractions were isolated from A549 cells. Total lystates, the cytosolic fraction, and mitochondria were analyzed by Western blotting (COXIV-mitochondria marker and α-tubulin-cytosolic marker). (B) A549 cells were transiently co-transfected with pEGFP-N1-CTMP and pDsRed-Mito for 24 h, and cells were observed using confocal microscopy. (C) A549 cells stably expressing pDsRed-Mito were infected with lentivirus-CTMP for 24 h and immunostained with anti-CTMP antibody. (D) The mitochondria fraction from A549 cells was isolated and treated with the indicated reagents. Samples were separated into supernatant (S) and precipitate (P) fractions, and then analyzed by immunoblotting with the indicated antibodies (cytochrome c, mitochondria membrane space protein; and COXIV, mitochondria membrane protein). (E) Immunofluorescence assay of apoptosis-inducing factor (AIF). A549 cells were grown on glass coverslips and transiently transfected with pEGFP-N1-CTMP. The cells were then stained with anti-AIF antibody (scale bar, 10 µm).
    Figure Legend Snippet: Figure 1. Localization of CTMP in mitochondria. (A) Cytoplasm and mitochondria fractions were isolated from A549 cells. Total lystates, the cytosolic fraction, and mitochondria were analyzed by Western blotting (COXIV-mitochondria marker and α-tubulin-cytosolic marker). (B) A549 cells were transiently co-transfected with pEGFP-N1-CTMP and pDsRed-Mito for 24 h, and cells were observed using confocal microscopy. (C) A549 cells stably expressing pDsRed-Mito were infected with lentivirus-CTMP for 24 h and immunostained with anti-CTMP antibody. (D) The mitochondria fraction from A549 cells was isolated and treated with the indicated reagents. Samples were separated into supernatant (S) and precipitate (P) fractions, and then analyzed by immunoblotting with the indicated antibodies (cytochrome c, mitochondria membrane space protein; and COXIV, mitochondria membrane protein). (E) Immunofluorescence assay of apoptosis-inducing factor (AIF). A549 cells were grown on glass coverslips and transiently transfected with pEGFP-N1-CTMP. The cells were then stained with anti-AIF antibody (scale bar, 10 µm).

    Techniques Used: Isolation, Western Blot, Marker, Transfection, Confocal Microscopy, Stable Transfection, Expressing, Infection, Membrane, Immunofluorescence, Staining

    Figure 2. CTMP induces apoptosis in A549 cells. (A) A549 cells were infected with lentivirus-CTMP for 24 h and treated with 1 µM staurosporine for the indi cated times (6 and 8 h). (B) A549 cells were infected with lentivirus-CTMP for 24 h and treated with 0.2 µM actinomycin D for the indicated times (12 and 24 h). (C) Western blot analysis was performed to measure CTMP expression in A549 cells after siRNA transfection for the indicated times. Right panel, densitometric analysis of CTMP expression in A549 cells after siRNA transfection for 48 h. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values and Western blot analysis measuring the levels of cleaved PARP and active caspase-3 was performed. (D) ELISA assay of active caspase-3. (E) ELISA assay of cleaved PARP. CON, untreated A549 cells; VEC, A549 cells infected with the lentivirus expression vector (VEC); CTMP, A549 cells infected with lentivirus-CTMP (CTMP). (F) Quantitative analysis of TUNEL-positive cells. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values.
    Figure Legend Snippet: Figure 2. CTMP induces apoptosis in A549 cells. (A) A549 cells were infected with lentivirus-CTMP for 24 h and treated with 1 µM staurosporine for the indi cated times (6 and 8 h). (B) A549 cells were infected with lentivirus-CTMP for 24 h and treated with 0.2 µM actinomycin D for the indicated times (12 and 24 h). (C) Western blot analysis was performed to measure CTMP expression in A549 cells after siRNA transfection for the indicated times. Right panel, densitometric analysis of CTMP expression in A549 cells after siRNA transfection for 48 h. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values and Western blot analysis measuring the levels of cleaved PARP and active caspase-3 was performed. (D) ELISA assay of active caspase-3. (E) ELISA assay of cleaved PARP. CON, untreated A549 cells; VEC, A549 cells infected with the lentivirus expression vector (VEC); CTMP, A549 cells infected with lentivirus-CTMP (CTMP). (F) Quantitative analysis of TUNEL-positive cells. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values.

    Techniques Used: Infection, Western Blot, Expressing, Transfection, Control, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, TUNEL Assay

    Figure 3. CTMP inhibits OPA1 expression and changes mitochondria morphology. (A) Western blot analysis of MFN1, MFN2, and DRP-1. A549 cells were infected with lentivirus-VEC/CTMP and cultured for 48 h before analysis. (B) A549 cells were infected with lentivirus-CTMP, treated with 0.2 µM CCCP (carbonyl cyanide 3-chlorophenylhydrazone), and transfected with siRNA specific for CTMP. (C) The mitochondrial ultrastructure was analyzed by TEM. (D) A549 cells stably expressing mitochondrion-targeted pDsRed-Mito were infected with lentivirus expression vector and lentivirus-CTMP for 48 h and observed with confocal microscopy (scale bar, 10 nm).
    Figure Legend Snippet: Figure 3. CTMP inhibits OPA1 expression and changes mitochondria morphology. (A) Western blot analysis of MFN1, MFN2, and DRP-1. A549 cells were infected with lentivirus-VEC/CTMP and cultured for 48 h before analysis. (B) A549 cells were infected with lentivirus-CTMP, treated with 0.2 µM CCCP (carbonyl cyanide 3-chlorophenylhydrazone), and transfected with siRNA specific for CTMP. (C) The mitochondrial ultrastructure was analyzed by TEM. (D) A549 cells stably expressing mitochondrion-targeted pDsRed-Mito were infected with lentivirus expression vector and lentivirus-CTMP for 48 h and observed with confocal microscopy (scale bar, 10 nm).

    Techniques Used: Expressing, Western Blot, Infection, Cell Culture, Transfection, Stable Transfection, Plasmid Preparation, Confocal Microscopy

    Figure 4. CTMP reduces mitochondria membrane potential that leads to cytochrome c release. (A) CTMP-infected cells were stained using 5 µg/ml JC-1 and analyzed by flow cytometry. (B) Western blot analysis of CTMP and cytochrome c in mitochondria. (C and D) The bands of interest were further analyzed by densitometry. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values.
    Figure Legend Snippet: Figure 4. CTMP reduces mitochondria membrane potential that leads to cytochrome c release. (A) CTMP-infected cells were stained using 5 µg/ml JC-1 and analyzed by flow cytometry. (B) Western blot analysis of CTMP and cytochrome c in mitochondria. (C and D) The bands of interest were further analyzed by densitometry. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values.

    Techniques Used: Membrane, Infection, Staining, Flow Cytometry, Western Blot, Control

    Figure 5. CTMP inhibits heat-shock proteins. A549 cells were infected with lentivirus-VEC/CTMP and cultured for 48 h. (A) Western blot analysis of Hsp27 and Apaf-1. (B) Immunofluorescence assay of Hsp27. (C) FACS analysis of Apaf-1 in CTMP-infected cells.
    Figure Legend Snippet: Figure 5. CTMP inhibits heat-shock proteins. A549 cells were infected with lentivirus-VEC/CTMP and cultured for 48 h. (A) Western blot analysis of Hsp27 and Apaf-1. (B) Immunofluorescence assay of Hsp27. (C) FACS analysis of Apaf-1 in CTMP-infected cells.

    Techniques Used: Infection, Cell Culture, Western Blot, Immunofluorescence

    Related Articles

    Synthesized:

    Article Title: Carboxyl-terminal modulator protein induces apoptosis by regulating mitochondrial function in lung cancer cells.
    Article Snippet: .. A549 cells were grown to 70% confluence, 10 nM of chemically synthesized siRNA targeting CTMP or non-targeting 20-25 nt siRNA (control siRNA) were transfected using Santa Cruz transfection reagent according to the manufacturer's instructions (Santa Cruz Biotechnology). ..

    Control:

    Article Title: Carboxyl-terminal modulator protein induces apoptosis by regulating mitochondrial function in lung cancer cells.
    Article Snippet: .. A549 cells were grown to 70% confluence, 10 nM of chemically synthesized siRNA targeting CTMP or non-targeting 20-25 nt siRNA (control siRNA) were transfected using Santa Cruz transfection reagent according to the manufacturer's instructions (Santa Cruz Biotechnology). ..

    Transfection:

    Article Title: Carboxyl-terminal modulator protein induces apoptosis by regulating mitochondrial function in lung cancer cells.
    Article Snippet: .. A549 cells were grown to 70% confluence, 10 nM of chemically synthesized siRNA targeting CTMP or non-targeting 20-25 nt siRNA (control siRNA) were transfected using Santa Cruz transfection reagent according to the manufacturer's instructions (Santa Cruz Biotechnology). ..



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    sirna targeting ctmp  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology sirna targeting ctmp
    Figure 1. Localization of <t>CTMP</t> in mitochondria. (A) Cytoplasm and mitochondria fractions were isolated from A549 cells. Total lystates, the cytosolic fraction, and mitochondria were analyzed by Western blotting (COXIV-mitochondria marker and α-tubulin-cytosolic marker). (B) A549 cells were transiently co-transfected with pEGFP-N1-CTMP and pDsRed-Mito for 24 h, and cells were observed using confocal microscopy. (C) A549 cells stably expressing pDsRed-Mito were infected with lentivirus-CTMP for 24 h and immunostained with anti-CTMP antibody. (D) The mitochondria fraction from A549 cells was isolated and treated with the indicated reagents. Samples were separated into supernatant (S) and precipitate (P) fractions, and then analyzed by immunoblotting with the indicated antibodies (cytochrome c, mitochondria membrane space protein; and COXIV, mitochondria membrane protein). (E) Immunofluorescence assay of apoptosis-inducing factor (AIF). A549 cells were grown on glass coverslips and transiently transfected with pEGFP-N1-CTMP. The cells were then stained with anti-AIF antibody (scale bar, 10 µm).
    Sirna Targeting Ctmp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sirna+targeting+ctmp/CTMP+siRNA/pm22200884-45-12-25
    Average 90 stars, based on 1 article reviews
    sirna targeting ctmp - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1. Localization of CTMP in mitochondria. (A) Cytoplasm and mitochondria fractions were isolated from A549 cells. Total lystates, the cytosolic fraction, and mitochondria were analyzed by Western blotting (COXIV-mitochondria marker and α-tubulin-cytosolic marker). (B) A549 cells were transiently co-transfected with pEGFP-N1-CTMP and pDsRed-Mito for 24 h, and cells were observed using confocal microscopy. (C) A549 cells stably expressing pDsRed-Mito were infected with lentivirus-CTMP for 24 h and immunostained with anti-CTMP antibody. (D) The mitochondria fraction from A549 cells was isolated and treated with the indicated reagents. Samples were separated into supernatant (S) and precipitate (P) fractions, and then analyzed by immunoblotting with the indicated antibodies (cytochrome c, mitochondria membrane space protein; and COXIV, mitochondria membrane protein). (E) Immunofluorescence assay of apoptosis-inducing factor (AIF). A549 cells were grown on glass coverslips and transiently transfected with pEGFP-N1-CTMP. The cells were then stained with anti-AIF antibody (scale bar, 10 µm).

    Journal: International journal of oncology

    Article Title: Carboxyl-terminal modulator protein induces apoptosis by regulating mitochondrial function in lung cancer cells.

    doi: 10.3892/ijo.2011.1319

    Figure Lengend Snippet: Figure 1. Localization of CTMP in mitochondria. (A) Cytoplasm and mitochondria fractions were isolated from A549 cells. Total lystates, the cytosolic fraction, and mitochondria were analyzed by Western blotting (COXIV-mitochondria marker and α-tubulin-cytosolic marker). (B) A549 cells were transiently co-transfected with pEGFP-N1-CTMP and pDsRed-Mito for 24 h, and cells were observed using confocal microscopy. (C) A549 cells stably expressing pDsRed-Mito were infected with lentivirus-CTMP for 24 h and immunostained with anti-CTMP antibody. (D) The mitochondria fraction from A549 cells was isolated and treated with the indicated reagents. Samples were separated into supernatant (S) and precipitate (P) fractions, and then analyzed by immunoblotting with the indicated antibodies (cytochrome c, mitochondria membrane space protein; and COXIV, mitochondria membrane protein). (E) Immunofluorescence assay of apoptosis-inducing factor (AIF). A549 cells were grown on glass coverslips and transiently transfected with pEGFP-N1-CTMP. The cells were then stained with anti-AIF antibody (scale bar, 10 µm).

    Article Snippet: A549 cells were grown to 70% confluence, 10 nM of chemically synthesized siRNA targeting CTMP or non-targeting 20-25 nt siRNA (control siRNA) were transfected using Santa Cruz transfection reagent according to the manufacturer's instructions (Santa Cruz Biotechnology).

    Techniques: Isolation, Western Blot, Marker, Transfection, Confocal Microscopy, Stable Transfection, Expressing, Infection, Membrane, Immunofluorescence, Staining

    Figure 2. CTMP induces apoptosis in A549 cells. (A) A549 cells were infected with lentivirus-CTMP for 24 h and treated with 1 µM staurosporine for the indi cated times (6 and 8 h). (B) A549 cells were infected with lentivirus-CTMP for 24 h and treated with 0.2 µM actinomycin D for the indicated times (12 and 24 h). (C) Western blot analysis was performed to measure CTMP expression in A549 cells after siRNA transfection for the indicated times. Right panel, densitometric analysis of CTMP expression in A549 cells after siRNA transfection for 48 h. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values and Western blot analysis measuring the levels of cleaved PARP and active caspase-3 was performed. (D) ELISA assay of active caspase-3. (E) ELISA assay of cleaved PARP. CON, untreated A549 cells; VEC, A549 cells infected with the lentivirus expression vector (VEC); CTMP, A549 cells infected with lentivirus-CTMP (CTMP). (F) Quantitative analysis of TUNEL-positive cells. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values.

    Journal: International journal of oncology

    Article Title: Carboxyl-terminal modulator protein induces apoptosis by regulating mitochondrial function in lung cancer cells.

    doi: 10.3892/ijo.2011.1319

    Figure Lengend Snippet: Figure 2. CTMP induces apoptosis in A549 cells. (A) A549 cells were infected with lentivirus-CTMP for 24 h and treated with 1 µM staurosporine for the indi cated times (6 and 8 h). (B) A549 cells were infected with lentivirus-CTMP for 24 h and treated with 0.2 µM actinomycin D for the indicated times (12 and 24 h). (C) Western blot analysis was performed to measure CTMP expression in A549 cells after siRNA transfection for the indicated times. Right panel, densitometric analysis of CTMP expression in A549 cells after siRNA transfection for 48 h. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values and Western blot analysis measuring the levels of cleaved PARP and active caspase-3 was performed. (D) ELISA assay of active caspase-3. (E) ELISA assay of cleaved PARP. CON, untreated A549 cells; VEC, A549 cells infected with the lentivirus expression vector (VEC); CTMP, A549 cells infected with lentivirus-CTMP (CTMP). (F) Quantitative analysis of TUNEL-positive cells. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values.

    Article Snippet: A549 cells were grown to 70% confluence, 10 nM of chemically synthesized siRNA targeting CTMP or non-targeting 20-25 nt siRNA (control siRNA) were transfected using Santa Cruz transfection reagent according to the manufacturer's instructions (Santa Cruz Biotechnology).

    Techniques: Infection, Western Blot, Expressing, Transfection, Control, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, TUNEL Assay

    Figure 3. CTMP inhibits OPA1 expression and changes mitochondria morphology. (A) Western blot analysis of MFN1, MFN2, and DRP-1. A549 cells were infected with lentivirus-VEC/CTMP and cultured for 48 h before analysis. (B) A549 cells were infected with lentivirus-CTMP, treated with 0.2 µM CCCP (carbonyl cyanide 3-chlorophenylhydrazone), and transfected with siRNA specific for CTMP. (C) The mitochondrial ultrastructure was analyzed by TEM. (D) A549 cells stably expressing mitochondrion-targeted pDsRed-Mito were infected with lentivirus expression vector and lentivirus-CTMP for 48 h and observed with confocal microscopy (scale bar, 10 nm).

    Journal: International journal of oncology

    Article Title: Carboxyl-terminal modulator protein induces apoptosis by regulating mitochondrial function in lung cancer cells.

    doi: 10.3892/ijo.2011.1319

    Figure Lengend Snippet: Figure 3. CTMP inhibits OPA1 expression and changes mitochondria morphology. (A) Western blot analysis of MFN1, MFN2, and DRP-1. A549 cells were infected with lentivirus-VEC/CTMP and cultured for 48 h before analysis. (B) A549 cells were infected with lentivirus-CTMP, treated with 0.2 µM CCCP (carbonyl cyanide 3-chlorophenylhydrazone), and transfected with siRNA specific for CTMP. (C) The mitochondrial ultrastructure was analyzed by TEM. (D) A549 cells stably expressing mitochondrion-targeted pDsRed-Mito were infected with lentivirus expression vector and lentivirus-CTMP for 48 h and observed with confocal microscopy (scale bar, 10 nm).

    Article Snippet: A549 cells were grown to 70% confluence, 10 nM of chemically synthesized siRNA targeting CTMP or non-targeting 20-25 nt siRNA (control siRNA) were transfected using Santa Cruz transfection reagent according to the manufacturer's instructions (Santa Cruz Biotechnology).

    Techniques: Expressing, Western Blot, Infection, Cell Culture, Transfection, Stable Transfection, Plasmid Preparation, Confocal Microscopy

    Figure 4. CTMP reduces mitochondria membrane potential that leads to cytochrome c release. (A) CTMP-infected cells were stained using 5 µg/ml JC-1 and analyzed by flow cytometry. (B) Western blot analysis of CTMP and cytochrome c in mitochondria. (C and D) The bands of interest were further analyzed by densitometry. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values.

    Journal: International journal of oncology

    Article Title: Carboxyl-terminal modulator protein induces apoptosis by regulating mitochondrial function in lung cancer cells.

    doi: 10.3892/ijo.2011.1319

    Figure Lengend Snippet: Figure 4. CTMP reduces mitochondria membrane potential that leads to cytochrome c release. (A) CTMP-infected cells were stained using 5 µg/ml JC-1 and analyzed by flow cytometry. (B) Western blot analysis of CTMP and cytochrome c in mitochondria. (C and D) The bands of interest were further analyzed by densitometry. Each bar represents the mean ± SE (n=3), *p<0.05 and **p<0.01 compared to the corresponding control values.

    Article Snippet: A549 cells were grown to 70% confluence, 10 nM of chemically synthesized siRNA targeting CTMP or non-targeting 20-25 nt siRNA (control siRNA) were transfected using Santa Cruz transfection reagent according to the manufacturer's instructions (Santa Cruz Biotechnology).

    Techniques: Membrane, Infection, Staining, Flow Cytometry, Western Blot, Control

    Figure 5. CTMP inhibits heat-shock proteins. A549 cells were infected with lentivirus-VEC/CTMP and cultured for 48 h. (A) Western blot analysis of Hsp27 and Apaf-1. (B) Immunofluorescence assay of Hsp27. (C) FACS analysis of Apaf-1 in CTMP-infected cells.

    Journal: International journal of oncology

    Article Title: Carboxyl-terminal modulator protein induces apoptosis by regulating mitochondrial function in lung cancer cells.

    doi: 10.3892/ijo.2011.1319

    Figure Lengend Snippet: Figure 5. CTMP inhibits heat-shock proteins. A549 cells were infected with lentivirus-VEC/CTMP and cultured for 48 h. (A) Western blot analysis of Hsp27 and Apaf-1. (B) Immunofluorescence assay of Hsp27. (C) FACS analysis of Apaf-1 in CTMP-infected cells.

    Article Snippet: A549 cells were grown to 70% confluence, 10 nM of chemically synthesized siRNA targeting CTMP or non-targeting 20-25 nt siRNA (control siRNA) were transfected using Santa Cruz transfection reagent according to the manufacturer's instructions (Santa Cruz Biotechnology).

    Techniques: Infection, Cell Culture, Western Blot, Immunofluorescence